Plasmid
pGEM-SBPas

Part:BBa_K4047039:Design

Designed by: An Nguyen   Group: iGEM21_MiamiU_OH   (2021-10-06)


pGEM-SBPase (plasmid for deletion of SBPase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1489
    Illegal BglII site found at 4478
    Illegal BamHI site found at 2345
    Illegal XhoI site found at 2433
    Illegal XhoI site found at 3675
    Illegal XhoI site found at 4474
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1998
    Illegal PstI site found at 2591
    Illegal AgeI site found at 1857
    Illegal AgeI site found at 4356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2430
    Illegal BsaI.rc site found at 864


Design Notes

Source

Upstream and downstream fragments of the gene sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase/ sedoheptulose-1,7-bisphosphatase class II at the locus tag Synpcc7942_0505

https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=491830&to=492867&report=fasta&strand=2

Backbone and components sourced from pXWK3-glgC Supplemental Table S2 from https://academic.oup.com/plphys/article/182/1/507/6116245

References